Molecular analysis of the function of direct repeats and a polypurine tract for plus-strand DNA priming in woodchuck hepatitis virus.

摘要: The replication of the hepadnavirus DNA genome is initiated by reverse transcription pregenome RNA into minus-strand followed plus-strand synthesis. priming requires transfer an primer from to primer-binding site on DNA. Annealing facilitated short direct repeats, DR1 and DR2. To investigate mechanism formation, we have introduced specific mutations DR2 measured effect these mutants initiation facilitate such analysis, constructed a vector for efficient expression woodchuck hepatitis virus in cultured cells. Our results suggest that 3' end determined prior its determination does not depend sequence motif at cleavage site. In addition, identified alternative synthesis purine-rich between Initiation this occurs independent repeats require