Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243

摘要: Phenotypic switching is characterized as a virulence factor of Candida spp. This study was carried out to evaluate the phenotypic ability C. krusei ATCC 14243 and determine its effect on biological properties, adherence capacity susceptibility towards chlorhexidine digluconate (CHX). To induce switched generations cultured under nitrogen-depleted growth conditions by adding phloxine B. These phenotypically colonies were designated 1st generation. Subsequent sub-culturing performed produce 2nd, 3rd 4th generations. The recovery generation highest at 85.7 while that lower 70.8, 2nd gradually reduced 46.6 36.4, respectively. All susceptible CHX. unswitched most but least adherent coated hard surfaces. susceptible, with ability. minimum inhibition concentration minimal fungicidal all determined 0.4 mg mL(-1). observations suggest activity induces changes properties