Acridine orange diagnosis of Plasmodium falciparum: evaluation after experimental infection.

摘要: The value and role of the acridine orange/microhematocrit tube method (quantitative buffy coat [QBC] analysis) in diagnosis malaria remains controversial. To establish true sensitivity this test comparison with thick blood film, we studied 49 subjects who were experimentally infected Plasmodium falciparum 10 vaccine infectivity trials. Diagnosis was made by orange staining 1-3 days earlier than film 23 (47%) at same time as 20. On other hand, six individuals. There no false positive results using among 584 specimens studied. a parasitemia less 11 parasites/microliters 65% cases. Where available, assay is clearly preferable terms speed accuracy to for parasitemias 150/microliters blood, perhaps important, ruling out infection P. symptomatic patient.