[4] Colorimetric ultramicro assay for reducing sugars
作者： Gad Avigad
DOI: 10.1016/S0076-6879(75)41006-0
关键词:
摘要: Publisher Summary This chapter describes the colorimetric ultramicro assay for reducing sugars. Ferrocyanide produced as result of reduction ferricyanide by a sugar is assayed indirectly formation ferrous ions. These subsequently interact with an iron chelate to yield product high color intensity. The reagent employed in following procedure 2,4,6-tripyridyl- s -triazine. Reactions should be shielded from direct light. Aliquots (1.0 ml) solutions containing 2–100 nmoles are placed test tubes. Reagent A added, and tubes kept 10 min boiling-water bath. B C (2.0 then added immediately, without cooling. stirred vigorously Vibromixer, after 5 dark, absorbance at 595 nm violet Fe (TPTZ) 2+ 2 against blank. It has been found out that 3-(2-pyridyl)-5,6-diphenyl-l,2,4-triazine can substitute TPTZ ions used determination ferrocyanide UV-spectrophotometry sugars described. method may only when no components other than adsorb significantly far ultraviolet range present solution analyzed.
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