Hydrolysis of phosphatidylcholine couples Ras to activation of Raf protein kinase during mitogenic signal transduction.
作者： H Cai , P Erhardt , J Troppmair , M T Diaz-Meco , G Sithanandam
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摘要: We have investigated the relationship between hydrolysis of phosphatidylcholine (PC) and activation Raf-1 protein kinase in Ras-mediated transduction mitogenic signals. As previously reported, cotransfection a PC-specific phospholipase C (PC-PLC) expression plasmid bypassed block to cell proliferation resulting from dominant inhibitory mutant Ras N-17. In contrast, PC-PLC failed bypass effect negative Raf mutants, suggesting that functions downstream but upstream Raf. Consistent with this hypothesis, treatment quiescent cells exogenous induced activation, even when normal function was blocked by N-17 expression. Further, response growth factors inhibition endogenous PC-PLC. Taken together, these results indicate PC mediates factors.
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