Prevalence and Fate of Carbapenemase Genes in a Wastewater Treatment Plant in Northern China.

摘要: Carbapenemase-producing strains of bacteria, which were primarily found in the medical field, have increasingly been environment, thus posing potential risks to public health. One possible way for carbapenemase genes enter environment is via wastewater. Therefore, goal this study was determine occurrence and fate five high-risk a wastewater treatment plant (WWTP) northern China using real-time qPCR. Results showed that blaKPC-2, blaGES-1, blaIMP-1 prevailed throughout all processing stages (even chlorination disinfection unit) WWTP, whereas blaVIM-2 blaOXA-48 not detected samples. Worryingly, considerable amounts ((1.54 ± 0.61) × 103 copies/mL (2.14± 0.41) 105 copies/mL) WWTP effluent samples, while majority transported dewatered sludge with concentrations from (6.51 0.14) 109 copies/g (6.18 0.63) 1010 dry weight. Furthermore, total 97 KPC-2-producing strains, belonging 8 bacterial genera, isolated WWTP. Sequencing 16S rRNA revealed most KPC-2 producing isolates opportunistic pathogens, including Klebsiella spp. (10.3%), Enterococcus (11.3%), Acinetobacter (19.6%), Escherichia (12.4%), Shigella (17.5%), Stenotrophomonas (10.3%) Wautersiella (9.3%). Moreover, blaKPC-2 identified first time Paenibacillus (an indigenous bacteria), indicating an increased risk horizontal transfer between clinical pathogens environmental bacteria. Indeed, conjugation experiment demonstrated gene E.coli J53 strain To our knowledge, obtain carrying quantify abundance environment.