The Highly Conserved Barley Powdery Mildew Effector BEC1019 Confers Susceptibility to Biotrophic and Necrotrophic Pathogens in Wheat.

摘要: Effector proteins secreted by plant pathogens play important roles in promoting colonization. Blumeria effector candidate (BEC) 1019, a highly conserved metalloprotease of graminis f. sp. hordei (Bgh), is essential for fungal haustorium formation, and silencing BEC1019 significantly reduces Bgh virulence. In this study, we found that homologs B. tritici (Bgt) Gaeumannomyces var. (Ggt) have complete sequence identity with those Bgh, prompting us to investigate their functions. Transcript levels were abundantly induced concomitant formation Bgt necrosis development Ggt-infected plants. overexpression considerably increased wheat susceptibility Ggt, whereas gene using host-induced enhanced resistance which was associated hydrogen peroxide accumulation, cell death, pathogenesis-related expression. Additionally, the full partial sequences can trigger death Nicotiana benthamiana leaves. These results indicate Ggt utilize as virulence promote colonization, thus these genes represent promising new targets breeding cultivars broad-spectrum resistance.