Novel amperometric flow-injection analysis of creatinine using a molecularly-imprinted polymer coated copper oxide nanoparticle-modified carbon-paste-electrode.

摘要: Abstract We report a novel amperometric flow-injection (FI) analysis of creatinine based on sensor comprising copper oxide nanoparticles (CuO) coated with molecularly-imprinted polymer (CuO@MIP) and decorating carbon-paste electrode (CPE) to form the CuO@MIP/CPE electrode. The CuO@MIP was synthesized by using CuO as supporting core, template, methacrylic acid (MAA) monomer, N, N′-(1,2-dihydroxyethylene)bis(acrylamide) (DHEBA) cross-linker, 2,2′-azobis (2-methylpropionitrile) (AIBN) initiator. Morphology structural characterization reveal that nanoparticle imprinted sites precipitation method, exhibits features are well suited detection: high surface area, good analyte diffusion adsorption characteristics provide shorter response times, large numbers specific cavities for enhanced capacity sensitivity. Cyclic voltammetric measurements indicate our provides excellent performance toward electro-oxidation creatinine. FI system used quantitatively determine at sensor, in phosphate buffer carrier. oxidation an applied potential +0.35 V flow rate 0.6 mL.min−1. as-prepared linear dynamic range detection from 0.5 200 μM (r2 = 0.995) limit 0.083 μM (S/N = 3). satisfactorily precision (%RSD = 1.94%, n = 30) selectivity There is only approximately 20% loss initial after 2 weeks when stored 4 oC. successfully detect human urine samples.