CD8+ T Cell and NK Responses to a Novel Dengue Epitope: A Possible Role for KIR3DL1 in Dengue Pathogenesis: A Dissertation

摘要: Variation in the sequence of T cell epitopes between dengue virus (DENV) serotypes is believed to alter memory responses during second heterologous infections contributing pathology following DENV infection. We identified a highly conserved, novel, HLA-B57-restricted epitope on NS1 protein, NS126-34. predicted higher frequencies NS126-34-specific CD8 + cells PBMC from individuals undergoing secondary, rather than primary, infection due expansion cells. generated tetramer against this (B57-NS126-34 TET) and used it assess phenotype antigen-specific samples clinical cohort children with acute established Bangkok, Thailand. High tetramer-positive were seen only 1 9 subjects secondary B57-NS126-34-specific, other epitope-specific cells, as well total expressed an activated (CD69 and/or CD38) In contrast, expression CD71 was largely limited DENV-specific vitro stimulation lines, three different epitopes, indicated that differentially sensitive by homologous variant peptides substantial upregulation detected which also elicited strong functional responses. may therefore represent useful marker antigenspecific activation.