Impact of pre-analytical factors on mycobacterium cultures contaminations rates in Burkina Faso, West Africa.

摘要: INTRODUCTION: for a high quality level diagnosis, mycobacterium culture must comply with the pre-analytical and analytical conditions recommended by WHO country National Tuberculosis Program (NTP). In this study, we determined whether temperature duration of sputum storage were associated contamination in Burkina Faso. METHODS: sputa collected 5 districts labs Faso. Temperature recorded. After collection, decontaminated using Petroff modified method, pellet was inoculated on LJ media supply 2% sodium pyruvate. Risk measured Chi2 test logistic regression. RESULTS: out 404 specimens, 61% (246/404) stored between 2 8°C, 15% (61/404) processed within three days. The global rate 24%, only 8% samples respecting recommendations, up to 35% others. Storage at room significantly higher risk compared 2-8°C (OR 2.24, p=0.001, IC 95%). CONCLUSION: recommendations about before cultures are not completely respected. This leads culture. It will be necessary take logistics measures peripherals health services or develop more selective medium low income countries.