Diversity in Butane Monooxygenases among Butane-Grown Bacteria

摘要: Butane monooxygenases of butane-grown Pseudomonas butanovora, Mycobacterium vaccae JOB5, and an environmental isolate, CF8, were compared at the physiological level. The presence butane in these bacteria was indicated by following results. (i) O(2) required for degradation. (ii) 1-Butanol produced during (iii) Acetylene inhibited both oxidation 1-butanol production. responses to known monooxygenase inactivator, ethylene, inhibitor, allyl thiourea (ATU), discriminated degradation among three bacteria. Ethylene irreversibly inactivated P. butanovora but not M. or CF8. In contrast, only CF8 strongly ATU. all strains bacteria, specific polypeptides labeled [(14)C]acetylene. [(14)C]acetylene labeling patterns different Exposure lactate-grown glucose-grown induced activity as well acetylene-binding polypeptides. Ammonia oxidized ammonia hydroxylamine, while nitrite. All ethylene oxide. Methane detected any results indicate distinct vaccae,