Measurement of human converting enzyme level by direct radioimmunoassay

摘要: CE was purified from human lung, and antisera were raised in rabbits. Antisera inhibited the activity of enzyme lung kidney plasma normal persons sarcoid patients. With at a titer 1:100,000, sensitive, direct RIA developed. or present gave parallel logit-log displacement lines, suggesting immunological identity. The level 400 +/- 131 ng/ml. In untreated patients, increased parallel. There negative correlation (r = -0.81) between diffusing capacity for CO Synthetic inhibitors such as captopril MK 421 did not interfere with RIA, permitting levels to be monitored patients undergoing acute inhibitor therapy. During administration 421, negligible change. contrast, renin eightfold during