Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene

摘要: Several cDNAs encoding the essential human splicing facor (SF) 1 have been cloned. Comparison of cDNA sequences suggested that corresponding mRNAs are generated by alternative from a common pre-mRNA. To confirm this assumption and to analyze possible modes used in generation these mRNAs, we determined structure gene SF1. The extends over approximately 15kb contains 14 exons. exon/intron at splice sites highly conserved mouse gene. SF1 is located on chromosome 11 close Menin, recently identified as responsible for multiple endocrine neoplasia-type (MEN1). absence TATA box 5' flanking region transcription unit suggests represents housekeeping However, genomic sequence analysis revealed putative binding regulatory factors upstream end cDNA. Analysis predicts use duplicated 3' well exon skipping intron inclusion generate six events.