Transcription factors under the control of the yeast Hog1 MAPK

摘要: Yeast cells are exposed to a wide variety of environment stresses, among them changes in the osmotic conditions. An osmolar upshift leads fast loose intracellular water, so living have developed mechanisms counteract this lost. In Saccharomyces cerevisiae conditions sensed by HOG pathway. The pathway is MAPK signalling and functional homolog stress activated JNK p38 present mammals. Because there high degree conservation these cascades, good model study adaptation processes. Recent reports shown that Hog1 can regulate several processes such as cell cycle control, metabolic or regulation gene expression. At beginning work, which was controlling expression were unclear because transcription factors under control not well characterized. Our goal identification new MAPK. Therefore, we designed genetic screen selected clones from multicopy genomic library able induce dependent genes non One SMP1 gene. Smp1 encodes for MEF2-like factor. Its overexpression induced osmoresponsive STL1, whereas smp1 defective their expression. showed reduced viability upon shock. Smp1-Hog1 interaction checked coprecipitation. Moreover, phosphorylated Hog1-dependent manner vitro phosphorylation experiments at C-terminal region. This important osmoadaptation functions. Moreover implicated adaptability stationary phase through Smp1. On other hand, microarrays studies HXT1 hexose transporter upregulated an shock manner. Expression gene, low affinity glucose cerevisiae, response general induction pathway, involving Snf3/Rgt2 membrane sensors, SCF-Grr1 ubiquitination complex Rgt1 addition found that, also requires Deletion components on both pathways results impaired Genetic analyses identified Sko1 factor modulating expression. Our here