Gene-specific sex effects on eosinophil infiltration in leishmaniasis

摘要: Sex influences susceptibility to many infectious diseases, including some manifestations of leishmaniasis. The disease is caused by parasites that enter the skin and can spread lymph nodes, spleen, liver, bone marrow, sometimes lungs. Parasites induce host defenses cell infiltration, leading protective or ineffective inflammation. These responses are often influenced genotype sex. We analyzed role sex in impact specific gene loci on eosinophil infiltration its functional relevance. studied genetic control eosinophils into inguinal nodes after 8 weeks Leishmania major infection using mouse strains BALB/c, STS, recombinant congenic CcS-1,-3,-4,-5,-7,-9,-11,-12,-15,-16,-18, -20, each which contains a different random set 12.5% genes from parental “donor” strain STS 87.5% “background” BALB/c. Numbers were counted hematoxylin-eosin-stained sections under light microscope. Parasite load was determined PCR-ELISA. resistant susceptible BALB/c mice contained very low intermediate numbers eosinophils, respectively. Unexpectedly, CcS-9 exceeded higher males than females. searched for controlling high linkage analysis F2 hybrids between detected four numbers. Lmr14 (chromosome 2) Lmr25 5) operate independently other (main effects). functions only males, effect independent. Lmr15 11) Lmr26 9) cooperation (non-additive interaction) with other. This interaction significant only, but sex-marker not significant. Eosinophil positively correlated parasite demonstrated strong influence L. present first identification sex-dependent autosomal eosinophilic infiltration. positive correlation suggests this reflects