Rational modular design of metabolic network for efficient production of plant polyphenol pinosylvin.

摘要: Efficient biosynthesis of the plant polyphenol pinosylvin, which has numerous applications in nutraceuticals and pharmaceuticals, is necessary to make biological production economically viable. To this end, an efficient Escherichia coli platform for pinosylvin was developed via a rational modular design approach. Initially, different candidate pathway enzymes were screened construct de novo directly from D-glucose. A comparative analysis intermediate pools identified that initial led cinnamic acid accumulation. The synthetic then divided into two new modules separated at acid. Combinatorial optimization transcriptional translational levels these resulted 16-fold increase titer. further improve concentration limiting precursor malonyl-CoA, malonyl-CoA synthesis module based on clustered regularly interspaced short palindromic repeats interference assembled optimized with other modules. final titer improved 281 mg/L, highest even D-glucose without any additional supplementation. approach described here could bolster our capabilities biology value-added chemical production.