摘要: Publisher Summary Class Gastropoda (snails, slugs, and their allies) is the most successful of molluskan classes as measured by ecological diversity. The earliest attempts to cultivate gastropod cells in vitro were done using either homologous hemolymph or simple artificial media support explanted tissues. More recently, during efforts establish cell lines from Biomphalaria glabrata, several investigators have attempted dissociate cells. Primary cultures kidney been reported for Cepaea memoralis, a land pulmonate snail. axenic cultivation endoparasites frequently difficult because fastidious needs organisms. development has proved be an expensive, time-consuming, frustrating endeavor. Neurobiologists utilized techniques study both secretory electrical activities neurons isolated central nervous system ganglia Aplysia, sea hare.