COMPOSITIONS AND METHODS USING METHANOTROPHIC S-LAYER PROTEINS FOR EXPRESSION OF HETEROLOGOUS PROTEINS

作者: Kalyuzhnaya Marina , Collins David , Demidenko Oleksandr

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摘要: In alternative embodiments, provided are compositions and methods for making a chimeric polypeptide comprising an S-layer heterologous or peptide. the comprise recombinantly engineering methylotrophic methanotrophic bacteria to express Also displaying immobilizing proteins on S-layer. recombinant assembled self-assembled isolated polypeptides. using bacteria, optionally Methylomicrobium alcaliphilum, M. alcaliphilum sp. 20Z, ectoine ((4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), production synthesis of protein, e.g., ectoine, enzyme, lipase.

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