Antibodies against oxidized phospholipids in laboratory tests exploring lupus anti-coagulant activity.

摘要: Lupus anti-coagulants (LA) are a variety of anti-phospholipid antibodies characterized by their capacity to interfere with phospholipid-dependent coagulation assays. LA increasingly recognized as important predictors thrombosis. However, the antigen specificity is still poorly characterized. Growing evidence indicates that oxidized phospholipids among targets antibodies. This prompted us investigate role IgG directed against different in 164 subjects without clotting factor defects were tested for presence using LA-sensitive activate partial thromboplastin time (aPTT-FSL) and screening/confirmation assay based on diluted Russell's viper venom test (dRVVT-PL). The response aPTT-FSL was significantly (P < 0.0005) associated high titres phosphatidylserine, phosphatidylethanolamine phosphatidylinositol, whereas positivity dRVVT-PL elevation phosphatidylcholine, phosphatidylinositol 0.01). No difference reactivity cardiolipin evident between groups. Positivity also 0.005) anti-cardiolipin anti-beta(2)-glycoprotein-1 IgG. stepwise logistic regression demonstrated recognizing phosphatidylcholine only independent assay, while test. In conclusion, autoantibodies defined detection or assays might contribute variability often observed responses functional tests detecting LA.