Modifications to improve the effectiveness of restriction fragment length polymorphism typing.

摘要: A streamlined and effective method for RFLP analysis of DNA has been developed. Southern transfers are accomplished by alkali blotting onto positively charged nylon membranes. The prehybridization step eliminated. hybridization solution is composed three cost-effective reagents: 7% SDS, 10% PEG, phosphate buffer. By using probes that hybridize to variable number tandem repeat loci, one two micrograms genomic can be achieved within five working days under normal hours. With longer autoradiographic exposures, as little 20-100 ng human sufficient analysis.