Pressure- and heat-induced protein unfolding in bacterial cells: crowding vs. sticking.
作者： Timothy Chen , Kapil Dave , Martin Gruebele
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摘要: In-cell protein stability is increased by crowding, but can be reduced destabilizing surface interactions. Will different denaturation techniques yield similar trends? Here, we apply pressure and thermal to green fluorescent protein/ReAsH-labeled yeast phosphoglycerate kinase (PGK) in Escherichia coli cells. Pressure more two state-like E. than vitro, stabilizing the native state. Thermal destabilizes PGK coli, unlike mammalian Results wild-type MG1655 strain are corroborated pressure-resistant J1 strain, where less prone aggregation. Thus, interactions overcome crowding cytoplasm under denaturation, not denaturation.
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