Characterization of miR-888 expression and regulation in endometrial cancer

摘要: Endometrial cancer is the fourth most common in women and gynecological malignancy. While patient outcome has improved for majority of cancers, outlook endometrial steadily decreased. In order to address this problem, we must better understand different mechanisms involved development progression. To end, quantified expression 667 miRNAs four endometrioid adenocarcinoma serous using Taqman Low Density Arrays (TLDAs). miR-888 was one highly overexpressed both subtypes. Analysis across multiple types The Cancer Genome Atlas database revealed that selectively expressed cancer, with a significant association invasive high grade tumors. addition, predominantly carcinosarcoma, rare but deadly form cancer. Therefore, conclude marks an aggressive tumor phenotype. One top predicted targets by TargetScan progesterone receptor (PR). PR potent suppressor endometrium whose often lost advanced cancers. We mRNA panel tumors found statistically significant, negative correlation between expression. Furthermore, overexpression cell lines capable decreasing at protein level. determine if directly PR, cloned each binding sites downstream Renilla luciferase into psiCHECK2 reporter vector.