Method of determining a viable count using a hydrophobic membrane filter

摘要: An improved method of determining a viable microbial cell count in sample solution with an enhanced detection sensitivity is provided, comprising filtering the through filtration membrane having hydrophobic properties to entrap microbes within barriers; applying thereto fine spray ATP releasing reagent extract luminescent ingredient from microbes; another liquid luminescence-inducing allow released emit luminescence; and measuring level luminescence, using competent means for luminescence level. The two types reagents are locally concentrated due thus greatly enhanced, permitting instant counting case large microbes, decreased culture time smaller bacterial microbes.