Compartmentation of Organic Acids in Corn Roots. III. Utilization of Exogenously Supplied Acids

摘要: The rates of utilization exogenously supplied (14)C labeled acids by corn roots was compared to the these generated endogenously in mitochondria from acetate-(3)H. (14)C-labeled citrate, pyruvate, succinate, glutamate or aspartate were with acetate-(3)H a 15 minute pulse and (3)H contents extracted measured over 4 hour period. It found, contrast previous experiments malate, that added used as rapidly endogenous forms. Apparently, therefore, penetrate readily into do not enter cytoplasmic pools which are ready equilibrium those mitochondria. Small amounts produced succinate-2,3-(3)H root tissue. Since would be expected reactions tricarboxylic acid cycle it concluded rather directly succinate. minor pool this way retained its radioactivity while lost. An extra-mitochondrial location is therefore suggested.