The novel human β-defensin 114 regulates lipopolysaccharide (LPS)-mediated inflammation and protects sperm from motility loss

摘要: Lipopolysaccharide (LPS) is an important pathological factor involved in serious inflammatory diseases and male reproductive impairments. Emerging evidence demonstrates that antimicrobial peptides possess protective activity response to LPS-induced inflammation. However, the LPS-binding and/or immunosuppressive of β-defensins (DEFBs) has been underestimated. In present work, we characterized a novel human defensin, DEFB114, which was expressed predominantly epididymis gingival cells at RNA level. Homogenous recombinant DEFB114 were prepared using mass spectrometry. protein exhibited broad spectrum with salt sensitivity against typical pathogenic microbes (i.e. Escherichia coli, Staphylococcus aureus, Candida albicans). Interestingly, demonstrated vitro inhibited TNF-α release RAW264.7 cultures through inhibition MAPK p42/44 when challenged LPS. Moreover, could also rescue reduction sperm motility protect d-galactosamine-sensitized C57BL/6 mice from lethality vivo. The on RAW264.7, sperm, disulfide bond-dependent because alkylated lost its activity. low cytotoxicity peptide toward erythrocytes indicative potential therapeutic use treatment inflammation, LPS contamination, potentially septic shock.