Effect of capsid confinement on the chromatin organization of the SV40 minichromosome
作者： Gadiel Saper , Stanislav Kler , Roi Asor , Ariella Oppenheim , Uri Raviv
DOI: 10.1093/NAR/GKS1270
关键词:
摘要: Using small-angle X-ray scattering, we determined the three-dimensional packing architecture of minichromosome confined within SV40 virus. In solution, minichromosome, composed closed circular dsDNA complexed in nucleosomes, was shown to be structurally similar cellular chromatin. contrast, find a unique organization nanometrically encapsidated chromatin, whereby minichromosomal density is somewhat higher at center capsid and decreases towards walls. This excellent agreement with coarse-grained computer model, accounting for tethered nucleosomal interactions under viral confinement. With analogy liquid crystals, but contrary solenoid structure our simulations indicate that nucleosomes lack orientational order. Nucleosomes layer adjacent wall, however, align boundary, thereby inducing ‘molten droplet’ state These findings suffice predict genome polyomavirus capsids underscore adaptable nature eukaryotic chromatin nanoscale
oup.com
paperity.org
oxfordjournals.org参考文章(90)
David H.J. Bunka, Stephen W. Lane, Claire L. Lane, Eric C. Dykeman, Robert J. Ford, Amy M. Barker, Reidun Twarock, Simon E.V. Phillips, Peter G. Stockley, Degenerate RNA packaging signals in the genome of Satellite Tobacco Necrosis Virus: implications for the assembly of a T=1 capsid. Journal of Molecular Biology. ,vol. 413, pp. 51- 65 ,(2011) , 10.1016/J.JMB.2011.07.063
Aurélie Bertin, Stéphanie Mangenot, Madalena Renouard, Dominique Durand, Françoise Livolant, Structure and Phase Diagram of Nucleosome Core Particles Aggregated by Multivalent Cations Biophysical Journal. ,vol. 93, pp. 3652- 3663 ,(2007) , 10.1529/BIOPHYSJ.107.108365
Tamar Schlick, Ognjen Perišić, Mesoscale Simulations of Two Nucleosome-Repeat Length Oligonucleosomes Physical Chemistry Chemical Physics. ,vol. 11, pp. 10729- 10737 ,(2009) , 10.1039/B918629H
Robert G. Martin, On the nucleoprotein core of simian virus 40 Virology. ,vol. 83, pp. 433- 437 ,(1977) , 10.1016/0042-6822(77)90190-8
Jennifer Galanis, Ralph Nossal, Wolfgang Losert, Daniel Harries, Nematic order in small systems: measuring the elastic and wall-anchoring constants in vibrofluidized granular rods. Physical Review Letters. ,vol. 105, pp. 168001- 168001 ,(2010) , 10.1103/PHYSREVLETT.105.168001
Frank Aumann, Jürgen Sühnel, Jörg Langowski, Stephan Diekmann, Rigid assembly and Monte Carlo models of stable and unstable chromatin structures: the effect of nucleosomal spacing Theoretical Chemistry Accounts. ,vol. 125, pp. 217- 231 ,(2010) , 10.1007/S00214-009-0561-9
J. Kindt, S. Tzlil, A. Ben-Shaul, W. M. Gelbart, DNA packaging and ejection forces in bacteriophage Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 13671- 13674 ,(2001) , 10.1073/PNAS.241486298
Aurélie Bertin, Amélie Leforestier, Dominique Durand, Françoise Livolant, Role of histone tails in the conformation and interactions of nucleosome core particles. Biochemistry. ,vol. 43, pp. 4773- 4780 ,(2004) , 10.1021/BI036210G
J.F. Diaz, J.M. Andreu, G. Diakun, E. Towns-Andrews, J. Bordas, Structural intermediates in the assembly of taxoid-induced microtubules and GDP-tubulin double rings: time-resolved X-ray scattering. Biophysical Journal. ,vol. 70, pp. 2408- 2420 ,(1996) , 10.1016/S0006-3495(96)79809-0
J. Sun, Q. Zhang, T. Schlick, Electrostatic mechanism of nucleosomal array folding revealed by computer simulation. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 102, pp. 8180- 8185 ,(2005) , 10.1073/PNAS.0408867102