Mechanical loading stimulates bone angiogenesis through enhancing type H vessel formation and downregulating exosomal miR-214-3p from bone marrow-derived mesenchymal stem cells.
作者： Xuetong Wang , Xinle Li , Jie Li , Lidong Zhai , Daquan Liu
关键词:
摘要: Exosomes are important transporters of miRNAs, which play varying roles in the healing bone fracture. Angiogenesis is one such critical events healing, and we previously reported stimulatory effect mechanical loading vessel remodeling. Focusing on type H vessels exosomal miR-214-3p, this study examined mechanism loading-driven angiogenesis. MiRNA sequencing qRT-PCR revealed that miR-214-3p was increased exosomes bone-losing ovariectomized (OVX) mice, while it significantly decreased by knee loading. Furthermore, compared to OVX group, exosomes, derived from promoted angiogenesis endothelial cells. In contrast, were transfected with angiogenic potential. Notably, improved microvascular volume, formation, mineral density contents, as well BV/TV, Tb.Th, Tb.N, Tb.Sp. cell cultures, overexpression cells reduced tube formation migration. Collectively, demonstrates promotes enhancing downregulating miR-214-3p.
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