Oxygen supply modulates MCP-1 release in monocytes from young and aged rats: decrease of MCP-1 transcription and translation is age-related.

摘要: Hyper or hypoxia may affect the immune system's chemokine production. Monocyte chemotactic protein-1 (MCP-1), an important cytokine can be activated by active oxygen species. Groups of rats were exposed to hypoxic and hyperoxic environmental conditions for 60 h MCP-1 was determined in their peripheral blood mononuclear cells Elisa Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). In this study we evaluated if ability monocytes produce under basal after stimulation with lipopolysaccharide (LPS) phytohaemagglutinin (PHA) differently affected exposure hyper young aged rats. expression production monocyte/macrophages from at normoxic reduced subjects. However, spontaneous, LPS PHA-induced up-regulated both (62 +/- 8, 99 7, 102 8 pg/ml, respectively) (79 4, 112 9, 117 10 pg/ ml, respectively). We conclude that hyperoxia is regulator release support hypothesis increased % O2 serve initiate which then serves recruit regulate distribution sites inflammation.