Hematopoietic and endothelial differentiation of human induced pluripotent stem cells.

摘要: Induced pluripotent stem cells (iPSCs) provide an unprecedented opportunity for modeling of human diseases in vitro, as well developing novel approaches regenerative therapy based on immunologically compatible cells. In this study, we employed OP9 differentiation system to characterize the hematopoietic and endothelial potential seven iPSC lines obtained from fetal, neonatal, adult fibroblasts through reprogramming with POU5F1, SOX2, NANOG, LIN28 compared it five embryonic cell (hESC, H1, H7, H9, H13, H14). Similar hESCs, all iPSCs generated CD34(+)CD43(+) progenitors CD31(+)CD43(-) coculture OP9. When cultured semisolid media presence growth factors, iPSC-derived primitive blood formed types colonies, including GEMM colony-forming Human induced (hiPSCs)-derived CD43(+) could be separated into following phenotypically defined subsets cells: CD43(+)CD235a(+)CD41a(+/-) (erythro-megakaryopoietic), lin(-)CD34(+)CD43(+)CD45(-) (multipotent), lin(-)CD34(+)CD43(+)CD45(+) (myeloid-skewed) Although observed some variations efficiency between different hiPSCs, pattern was very similar tested or three four genes. several issues remain resolved before can administered humans therapeutic purposes, patient-specific already used characterization mechanisms identification molecules that correct affected genetic networks.