Mapping brain glucose uptake with chemical exchange-sensitive spin-lock magnetic resonance imaging.

摘要: Uptake of administered D-glucose (Glc) or 2-deoxy-D-glucose (2DG) has been indirectly mapped through the chemical exchange (CE) between glucose hydroxyl and water protons using CE-dependent saturation transfer (glucoCEST) magnetic resonance imaging (MRI). We propose an alternative technique—on-resonance CE-sensitive spin-lock (CESL) MRI—to enhance responses to changes. Phantom data simulations suggest higher sensitivity for this ‘glucoCESL’ technique (versus glucoCEST) in intermediate CE regime relevant glucose. Simulations CESL signals also show insensitivity B0-fluctuations. Several findings are apparent from vivo glucoCESL studies rat brain at 9.4 Tesla with intravenous injections. First, dose-dependent nearly linearly 0.25-, 0.5-, 1-g/kg Glc administration (obtained 12-second temporal resolution), changes robustly detected all doses. Second, a matched dose 1 g/kg much larger persist longer duration 2DG versus administration, minimal mannitol as osmolality control. And third, similar increases steady-state blood levels, ~2.2 times Glc, consistent their different metabolic properties. Overall, we that MRI could be highly sensitive quantifiable tool transport metabolism studies.