Melatonin antagonizes lipopolysaccharide-induced pulpal fibroblast responses.

摘要: Pulpal inflammation is known to be mediated by multiple signaling pathways. However, whether melatonin plays regulatory roles in pulpal remains unclear. This study aimed at elucidating an situ expression of and its receptors human tissues, the contribution on antagonism lipopolysaccharide (LPS)-infected fibroblasts. Melatonin tissues harvested from healthy teeth was investigated immunohistochemical staining. Its receptors, receptor 1 (MT1) 2 (MT2), were also immunostained isolated inflamed diagnosed with irreversible pulpitis. Morphometric analysis subsequently performed. After LPS infection cultured fibroblasts, cyclo-oxygenase (COX) interleukin-1 β (IL-1 β) transcripts examined using reverse transcription-polymerase chain reaction (RT-PCR). Analysis mRNA performed investigate stimulation via COX IL-1 induction. Mann-Whitney U test One-way ANOVA used for statistical determine a significance level. expressed tissue within odontoblastic zone, cell-rich connective tissue. Furthermore, health, strong MT1 MT2 distributed similarly all 3 zones. In contrast, during disease, reduced (P-value< 0.001), but not (P-value = 0.559). Co-culturing resulted reduction COX-2 primary indicating that may play antagonistic role Human dental pulp abundantly layers layers. exerted activity against LPS-mediated induction suggesting therapeutic potential possible immunomodulation functional pulp.