Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines

摘要: The aim of this paper is to analyse sunitinib malate in vitro ability enhance cisplatin cytotoxicity T24, 5637, and HT1376 human urinary bladder-cancer cell lines. Cells were treated with (3, 6, 13, 18 μM) (1, 2, 4, 20 μM), either isolation or combined, over the course 72 hours. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, monodansylcadaverine staining flow cytometry performed. combination index (CI) was calculated based on Chou Talalay method. In isolation, statistically (P < 0.05) decrease viability all lines a dose-dependent manner, presence autophagic vacuoles. A cycle arrest early S-phase G0/G1-phase also found after exposure malate, respectively. Treatment cells showed synergistic effect (CI 1). Autophagy apoptosis studies greater incidence when combined treatment put into use. This hints at possibility new therapeutic approach. If confirmed vivo, conjugation may provide means perspectives muscle-invasive bladder cancer treatment.