PAF-acether induced arterial thrombosis and the effect of specific antagonists.

摘要: Platelet-vessel wall interactions and local thrombosis are investigated in vivo a branch of the mesenteric artery guinea pig, using optoelectronic registration ultrastructural control. Following an electrical challenge resulting changes cell membrane polarization, subsequent superfusion by PAF-acether or stable analogue, (1-O-alkyl-2-N-methylcarbamyl-sn-glycero-3-phosphocholine, 10(-8) M focal concentration (f.c.)) for restricted period results endothelial retraction bleb formation followed platelet adhesion development thrombus which over time becomes invaded leukocytes eventually occludes vascular lumen. It was demonstrated previous investigation that these phenomena triggered generation endogenous cells. Specific PAF-acether-antagonists, such as BN 52021 ginkgolide, but also synthetic molecules, derivatives triazolo-pyridino-diazepine group (BN 50727, 50755 50789), significantly inhibit platelet-vessel thrombosis, not blebs Hydrogen peroxide (10(-5)M f.c.) only primes effect PAF-acether, is itself capable inducing through PAF-acether-mediated mechanism. Inhibition acetyl hydrolase PMSF (phenyl-methyl-sulfonyl-fluoride, 10(-5)M invariably significant enhancement while conversely, inhibition transferase 27584 RP (4-(naphtylvinyl)pyridine hydrochloride, 10(-6)M inhibits thromboformation indicating remodeling pathway involved.