A systematic review of the asymmetric inheritance of cellular organelles in eukaryotes: A critique of basic science validity and imprecision.

摘要: We performed a systematic review to identify all original publications describing the asymmetric inheritance of cellular organelles in normal animal eukaryotic cells and critique validity imprecision evidence. Searches were Embase, MEDLINE Pubmed up November 2015. Screening titles, abstracts full papers was by two independent reviewers. Data extraction one reviewer checked second reviewer. Study quality assessed using SYRCLE risk bias tool, for studies developing tools experimental model, organelle markers imprecision. A narrative data synthesis performed. identified 31 (34 publications) after mitotic or meiotic division. Studies centrosomes (n = 9); endosomes 6), P granules 4), midbody 3), mitochondria proteosomes 2), spectrosomes cilia 2) endoplasmic reticulum identified. Asymmetry defined quantified variable methods. Assessment statistical reliability results indicated only (7%) judged have low concern, majority (77%) 'unclear' five (16%) 'high concerns'; main reasons technical repeats (<10). model that (61%) be valid, ten (32%) unclear both described 'stem cells' without providing evidence confirm this (pluripotency self-renewal). marker no had most (96.5%), indicating there insufficient details judge if appropriate. One study high concern due contradictory same organelle. For could not confirmed. In particular, limited lack reporting interassay variability, sample size calculations, controls functional validation markers. An evaluation 16 reviews containing cell assays found 50% reported adherence PRISMA ARRIVE guidelines 38% formal assessment. 44% did consider how relevant valid models research question. 75% were. 69% impact results. Future basic preclinical should ensure rigorous addition Increased awareness importance is needed scientific community.