Action-Self Quenching: Dimer-Induced Fluorescence Quenching of Chromophores as a Probe for Biomolecular Structure

摘要: To obtain a more detailed understanding of how structure influences the function and interaction biomolecules, it is important to develop sensitive techniques probe these relationships. Alongside in vivo vitro techniques, instructive consider vacuo methodologies: for example native mass spectrometry, ion mobility FRET. Here, we propose novel technique probing biomolecular based on changes photophysics chromophore upon dimer formation. Comparison solution gas phase measurements doubly tagged tripeptide shows that dimer-induced fluorescence quenching accompanied by an increase photofragmentation yield. The 12–28 fragment amyloid beta was used show as charge state increased—previously shown cause conformational change from compact random coil extended helical structure—the disappearance band at 495 nm could be correlated with level self-quenching. presence feat...