A hypothesis of the disc-sphere transformation of the erythrocytes between glass surfaces and of related observations.

摘要: Erythrocytes suspended at a low hematocrit in non-buffered isotonic saline change from biconcave discs to spheres between glass surfaces of slide and coverslip with the echinocyte as an intermediate. A pH increase is major factor responsible for this disc-sphere transformation or effect. It also observed made various polymers mica provided that distance them controlled (0.1 mm). The effect antagonized by serum, plasma, serum albumin, ammonium salts CO2. not above 1-2% hematocrit, but enhanced gamma-globulins. sites reappearance spicules are same order their disappearance inverse during repetitive cycle reversal when small rod alternatively approached near site on erythrocyte surface withdrawn. mechanism shape control has been previously hypothesized which Band 3 (AE1), anion exchange protein, plays central role. Specifically, decrease ratio its outward-facing conformation (Band 3o) inward-facing 3i) contract relax membrane skeleton, promoting echinocytosis stomatocytosis, respectively. 3o/Band 3i equilibrium determined Donnan Cl-, HCO3- H+ (r=Cl(i)-/Cl(o)-=HCO3i-/HCO3o-=Ho+/Hi+), increasing it. could explain observations assumptions permeable CO2 unstirred layer slows propagation occurring approach peripheral sites. presence plasma may be basis absence these fluids.