Acoustic-spectrum-compensated photoacoustic microscopy

摘要: Photoacoustic microscopy (PAM) can label-free image oxy- and deoxy-hemoglobin (${{\rm HbO}_2}$HbO2 Hb) concentrations in vivo, providing useful information for metabolic researches diagnostic applications. Conventional PAM assumes a linear relationship between the photoacoustic amplitude absorption coefficient. However, many factors, including absorber size, laser pulse width, frequency response of ultrasound transducer, may affect measured acoustic spectrum shape temporal signal. The transducer weigh blood vessels differently according to their diameters. In addition, width also affects signal amplitude. These factors cause inaccurate measurement Hb ${{\rm concentrations. To address this issue, we develop an acoustic-spectrum-compensated optical-resolution (OR-PAM) that corrects nonuniform makes quantitative results be independent vessel diameter width. dual-wavelength OR-PAM, demonstrate compensation improve accuracy oxygen saturation imaging by $\sim{15}\% $∼15%.