Protocol for high-resolution separation of rodent myosin heavy chain isoforms in a mini-gel electrophoresis system.
作者： Wataru Mizunoya , Jun-ichiro Wakamatsu , Ryuichi Tatsumi , Yoshihide Ikeuchi
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摘要: Skeletal muscle comprises several fiber types classified based on their contractile and metabolic properties. are according to myosin heavy chain isoforms (MyHC I, IIa, IIx, IIb). We attained good separation of MyHC in a mini-gel system by modifying previously developed electrophoresis protocol. Increased glycerol decreased cross-linking agent concentrations improved the isoforms. Sample preparation with dithiothreitol protease inhibitors produced clear band boundaries. This protocol included silver staining, linear range. The provided high resolution highly accurate assay rodent
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