Metal Ion Interactions with Apo-Concanavalin A and Some Observations on Metal Ion Requirements and Sugar Binding byBandeiraea simplicifoliaI Lectin

摘要: The kinetics of interaction Ca2+ ions with M ConA (M = Co2+, Mn2+, Ni2+ and Zn2+) were followed by using the quenching fluorescence 4-methylumbelliferyl-α-D-mannopyranoside when bound to protein. kinetic data can be interpreted in terms a mechanism which species (MCaP)1 initially formed (constant K1) transforms slowly (k2) final form. Values k1, ΔH1 ΔS1 (particularly) k2, ΔH2≠ ΔS2≠ are very similar for all metals, but ΔH2≠-values much lower than previously reported other investigators. binding 4-methylumbelliferyl-α-D-galactopyranoside Bandeiraea simplicifolia I lectin studied stopped-flow methods. formation rate constant (∼ 105 M−1 s−1) is that sugars lectins. EDTA removes sugar ability B. t1/2 24 min (pH 7.2, 25°). addition apolectin give product appears controlled conformational change.