The internal pH of the forespore compartment of Bacillus megaterium decreases by about 1 pH unit during sporulation.

摘要: Previous work has shown that the internal pH of dormant spores Bacillus species is more than 1 U below growing cells but rises to in first minutes spore germination. In present whole megaterium sporangium was measured by distribution weak base methylamine and found decrease approximately 0.4 during sporulation. By using fluorescence ratio image analysis with a fluorescein derivative, 2',7'-bis(2-carboxyethyl)-5 (and -6)-carboxyfluorescein (BCECF), whose sensitive, mother cell remain constant sporulation at value 8.1, similar vegetative cell. Whereas forespore initially this fell 7.0 90 min before synthesis dipicolinic acid well accumulation depot 3-phosphoglyceric acid. The compartment brought suspending sporulating 8 potassium phosphate buffer plus ionophore nigericin clamp external medium. We suggest minimum, acidification may regulate activity phosphoglycerate mutase, which enzyme known be regulated allow