Dopant Enriched Nitrogen Gas Combined with Sheathless Capillary Electrophoresis–Electrospray Ionization-Mass Spectrometry for Improved Sensitivity and Repeatability in Glycopeptide Analysis
作者： Guinevere S. M. Kammeijer , Isabelle Kohler , Bas C. Jansen , Paul J. Hensbergen , Oleg A. Mayboroda
DOI: 10.1021/ACS.ANALCHEM.6B00479
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摘要: Over the last years, numerous strategies have been proposed to enhance both ionization efficiency and spray stability in electrospray (ESI), particular for nanospray applications. In nano-liquid chromatography–mass spectrometry (nano-LC–ESI-MS), a better ESI performance has observed when coaxial gas flow is added around emitter. Moreover, enrichment of with an organic dopant led improved desolvation overall enhanced sensitivity. this study, use enriched nitrogen (DEN)-gas combined sheathless capillary electrophoresis (CE)–ESI-MS was evaluated glycopeptide analysis. Using acetonitrile as dopant, increased sensitivity compared conventional CE–ESI-MS. Up 25-fold higher sensitivities model glycopeptides were obtained, allowing limits detection unachieved by state-of-the-art nano-LC–ESI-MS. The effect DEN-gas on repeatability intermediate precisio...
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