Efficient inhibition of hepatitis B virus infection by acylated peptides derived from the large viral surface protein.

摘要: The lack of an appropriate in vitro infection system for the major human pathogen hepatitis B virus (HBV) has prevented a molecular understanding early events HBV. We used novel HBV-infectible cell line HepaRG and primary hepatocytes to investigate interference by HBV envelope protein-derived peptides. found that peptide consisting authentically myristoylated N-terminal 47 amino acids pre-S1 domain large viral protein (L protein) specifically infection, with 50% inhibitory concentration (IC50) 8 nM. replacement myristic acid other hydrophobic moieties resulted changes activity, most notably decrease IC50 picomolar concentrations longer unbranched fatty acids. obstruction susceptibility after short preincubation times peptides suggested efficiently target inactivate receptor at hepatocyte surface. Our data both shed light on mechanism entry into provide basis development potent hepadnaviral inhibitors as therapeutic concept treatment Beta.