Renal allograft histopathology in dog leukocyte antigen mismatched dogs after renal transplantation.

摘要: Objective— To evaluate allograft histopathology in dog leukocyte antigen (DLA)-mismatched dogs undergoing renal transplantation, with transient immunosuppression. Study Design— Prospective study. Animals— Ten healthy adult mongrel dogs. Methods— Reciprocal transplantation and bilateral nephrectomy were performed. Immune conditioning consisted of nonmyeloablative (200 cGy), total body irradiation (TBI), bone marrow (BMT; 7 dogs), cyclosporine (CSA; 15 mg/kg every 12 hours), mycophenolate mofetil (MMF; 10 mg/kg hours) intermittent prednisone (1 mg/kg 12–24 hours). Biopsies collected at during full immunosuppression (44–90 days), once medications reduced or discontinued (228–580 days). evaluated for interstitial, tubular, vascular, glomerular lesions. Blood urea nitrogen, creatinine, serum CSA concentrations, clinical score determined each biopsy. Results— Seven survived >200 days (mean, 380 Transient toxicity was suspected 6 dogs. Lymphocytic, plasmacytic interstitial inflammation, tubulitis progressed when immunosuppressive decreased. All had histologic lesions consistent some degree rejection study end. Conclusion— Nonmyeloablative TBI, BMT, short-term CSA, MMF, allowed function survival days. It appears unlikely that drug withdrawal will be possible unrelated DLA-mismatched using this protocol. Clinical Relevance— along BMT TBI may make kidney a reality treatment failure Initiating both MMF lower dosages potentially eliminate early injury.