Enhanced Th1 and Th17 responses in peripheral blood in active non-segmental vitiligo

摘要: Accumulating studies have indicated that vitiligo, especially non-segmental vitiligo (NSV), is one kind of autoimmune diseases and CD4+ T cells play important roles in the pathogenesis. However, there been very limited data on detailed changes each CD4+ T cell subsets periphery active NSV. To clarify this issue, we collected peripheral blood mononuclear (PBMCs) from 30 patients with NSV age- sex-matched healthy controls. The percentages circulating Th1, Th2, Th17 Tregs were evaluated using flow cytometry expressions their specific transcription factors T-bet, GATA3, RORγt FOXP3 at mRNA level protein levels qualified by qPCR cytometry, respectively. Meanwhile, expression IFN-γ, IL-4, TGF-β, IL-17A serum measured. We found NSV, absolute numbers Th1 both significantly higher than those controls, while Th2 showed no significant difference compared to Moreover, ratios Th1/Tregs Th17/Tregs circulation statistically elevated Similar results got qualification corresponding levels. Compared was increased productions TGF-β had change. These suggested subsets, played major role cellular immunity progression vitiligo. immune lever inclined effector not suppressor may result loss self-tolerance melanocytes.