A ruthenium probe for cell viability measurement using flow cytometry, confocal microscopy and time-resolved luminescence.
作者： M. Emilia Jiménez-Hernández , Guillermo Orellana , Francisco Montero , M. Teresa Portolés
DOI: 10.1562/0031-8655(2000)072<0028:ARPFCV>2.0.CO;2
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摘要: Abstract The capability of the new luminescent probe (dibenzo[h,j]dipyrido[3,2-a:2′,3′-c]phenazine)bis(2,2′-bipyridine)ruthenium(II) dication, (RB2Z), to discriminate live and dead cells has been tested on rat hepatocytes mouse lymphocytes. RB2Z-stained were analyzed using flow cytometry, fluorescence (confocal) microscopy time-resolved luminescence measurements. established viability probes propidium iodide (PI) SYTOX® green (SG) used as controls. intense RB2Z at 606 nm is localized in nucleus nonviable cells. Viability measurements by cytometry dead-cell marker yield same results PI SG. lifetime also displays sensitivity cell (0.45 0.82 μs presence fully viable cells, respectively). This ruthenium complex photostable under laser sources its 200 Stokes shift facilitates multicolor labeling experiments fluor...
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