[8] Single vertical spin density gradient ultracentrifugation
作者： Byung H. Chung , Jere P. Segrest , Marjorie J. Ray , John D. Brunzell , John E. Hokanson
DOI: 10.1016/0076-6879(86)28068-4
关键词:
摘要: Publisher Summary Density gradient ultracentrifugation in the swing out rotor has been successfully applied to fractionation of major lipoprotein species and their subspecies; however, it requires a minimum 24–36 h complete separation fractions plasma. This chapter describes 13 separate vertical spin procedures for preparative number different plasma lipoproteins method quantitative analysis cholesterol from separated by single (SVS) density ultracentrifugation. The works on principle that compression geometry when at speed shortens time over more conventional techniques without loss resolution. procedure advantages methods including decreased time, degradation, good But there are certain disadvantages this also, such as wall adherence VLDL albumin, resolution, volume limit per rotor. However, addresses solutions problems angled-head rotors.
elsevier.com
sci-hub.se 参考文章(13)
Anthony H.M. Terpstra, Christopher J.H. Woodward, Francisco J. Sanchez-Muniz, Improved techniques for the separation of serum lipoproteins by density gradient ultracentrifugation: Visualization by prestaining and rapid separation of serum lipoproteins from small volumes of serum Analytical Biochemistry. ,vol. 111, pp. 149- 157 ,(1981) , 10.1016/0003-2697(81)90243-8
Richard H. Hintox, Axdrzej J. Kowalski, Alan Mallinson, Choice of conditions for the gradient flotation of serum lipoproteins in swing-out rotors. Clinica Chimica Acta. ,vol. 44, pp. 267- 270 ,(1973) , 10.1016/0009-8981(73)90391-4
F. T. Lindgren, L. C. Jensen, R. D. Wills, G. R. Stevens, Subfractionation of Sf 4–105, Sf 4–20 and high density lipoproteins Lipids. ,vol. 7, pp. 194- 201 ,(1972) , 10.1007/BF02533063
B H Chung, T Wilkinson, J C Geer, J P Segrest, Preparative and quantitative isolation of plasma lipoproteins: rapid, single discontinuous density gradient ultracentrifugation in a vertical rotor. Journal of Lipid Research. ,vol. 21, pp. 284- 291 ,(1980) , 10.1016/S0022-2275(20)39807-2
Richard J. Havel, Howard A. Eder, Joseph H. Bragdon, THE DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUM Journal of Clinical Investigation. ,vol. 34, pp. 1345- 1353 ,(1955) , 10.1172/JCI103182
B H Chung, J P Segrest, J T Cone, J Pfau, J C Geer, L A Duncan, High resolution plasma lipoprotein cholesterol profiles by a rapid, high volume semi-automated method. Journal of Lipid Research. ,vol. 22, pp. 1003- 1014 ,(1981) , 10.1016/S0022-2275(20)37338-7
J R Foreman, J B Karlin, C Edelstein, D J Juhn, A H Rubenstein, A M Scanu, Fractionation of human serum lipoproteins by single-spin gradient ultracentrifugation: quantification of apolipoproteins B and A-I and lipid components Journal of Lipid Research. ,vol. 18, pp. 759- 767 ,(1977) , 10.1016/S0022-2275(20)41594-9
R M Krauss, D J Burke, Identification of multiple subclasses of plasma low density lipoproteins in normal humans Journal of Lipid Research. ,vol. 23, pp. 97- 104 ,(1982) , 10.1016/S0022-2275(20)38178-5
Trudy M. Forte, Ronald M. Krauss, Mason M. S. Shen, Frank T. Lindgren, Heterogeneity of serum low density lipoproteins in normal human subjects Journal of Lipid Research. ,vol. 22, pp. 236- 244 ,(1981) , 10.1016/S0022-2275(20)35367-0
M J Chapman, S Goldstein, D Lagrange, P M Laplaud, A density gradient ultracentrifugal procedure for the isolation of the major lipoprotein classes from human serum. Journal of Lipid Research. ,vol. 22, pp. 339- 358 ,(1981) , 10.1016/S0022-2275(20)35376-1