Acute Effects of Butyrate on Induced Hyperpermeability and Tight Junction Protein Expression in Human Colonic Tissues.

摘要: Intact intestinal barrier function is essential for maintaining homeostasis. A dysfunctional can lead to local and systemic inflammation through translocation of luminal antigens has been associated with a range health disorders. Butyrate, short-chain fatty acid derived from microbial fermentation dietary fibers in the colon, described as an barrier-strengthening agent, although mainly by using vitro animal models. This study aimed investigate butyrate's ability prevent hyperpermeability, induced mast cell degranulator Compound 48/80 (C48/80), human colonic tissues. Colonic biopsies were collected 16 healthy subjects permeability was assessed Ussing chamber experiments. Furthermore, expression levels tight junction-related proteins determined quantitative reverse transcription polymerase chain reaction (qRT-PCR). Pre-treatment 5 mM butyrate or 25 did not protect tissue against paracellular transcellular measured FITC-dextran horseradish peroxidase passage, respectively. Biopsies treated prior stimulation C48/80 showed reduced claudin 1. In conclusion, this translational ex vivo demonstrate acute protective effect chemical insult humans.