Enhanced reducing sugar production by saccharification of lignocellulosic biomass, Pennisetum species through cellulase from a newly isolated Aspergillus fumigatus.
作者： Sonali Mohapatra , Sameer Padhy , Pradeep K. Das Mohapatra , H.N. Thatoi
DOI: 10.1016/J.BIORTECH.2018.01.023
关键词:
摘要: Abstract A cellulose degrading fungus Aspergillus fumigatus (CWSF-7) isolated from decomposed lignocellulosic waste containing soil was found to produce high titer of cellulases. The optimum activity CMCase and FPase were 1.9 U/mL 0.9 U/mL respectively while the highest protein concentration be 1.2 mg/mL. Saccharification two Pennisetum grass varieties [dennanath (DG) hybrid napier (HNG)] optimized using partially purified in equal concentration, i.e. a ratios 1:1 further with addition commercial xylanase response surface methodology (RSM). production total reducing sugar (TRS) cellulase 396.6 355.8 (mg/g), whereas had higher TRS titers 478.7 483.3 (mg/g) for DG HNG as evident HPLC analysis. Further, characterization enzyme saccharified by SEM ATR-FTIR revealed efficient hydrolysis hemicellulose.
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