Developing an Articular Cartilage Decellularization Process Toward Facet Joint Cartilage Replacement

摘要: OBJECTIVE: The facet joint has been identified as a significant source of morbidity in lower back pain. In general, treatments have focused on reducing the pain associated with osteoarthritis, and no targeted development replacement tissue for arthritic articular cartilage. Therefore, objective this study was to develop nonimmunogenic decellularized cartilage while maintaining functional properties similar native tissue. METHODS: vitro testing performed bovine explants. effects 2% sodium dodecyl sulfate (SDS), detergent used cell nuclear membrane solubilization, cellularity, biochemical, biomechanical properties, were examined. Compressive determined using creep indentation, tensile obtained uniaxial testing. Biochemical assessment involved determination DNA content, glycosaminoglycan (GAG) collagen content. Histological examination included hematoxylin eosin staining well GAG. RESULTS: Treatment SDS 2 hours maintained compressive GAG content resulting decrease nuclei 4% Additionally, treatment 8 resulted complete histological decellularization 40% properties. However, observed. Similar results observed 4 treatment, although not great treatment. CONCLUSION: decreased mechanical whereas but had minimal effect future studies must be optimize close those This served step creating that could osteoarthritis.