SC1 Promotes MiR124-3p Expression to Maintain the Self-Renewal of Mouse Embryonic Stem Cells by Inhibiting the MEK/ERK Pathway.

摘要: Background/Aims: Self-renewal is one of the most important features embryonic stem (ES) cells. SC1 a small molecule modulator that effectively maintains self-renewal mouse ES cells in absence leukemia inhibitory factor (LIF), serum and feeder However, mechanism by which undifferentiated state remains unclear. Methods: In this study, microarray RNA deep-sequencing experiments were performed on treated with or without to identify key genes microRNAs contributed self-renewal. Results: regulates expressions pluripotency differentiation factors, antagonizes retinoic acid (RA)-induced presence LIF. inhibits MEK/ERK pathway through Kyoto Encyclopedia Genes Genomes (KEGG) analysis reporting experiments. Small revealed significantly modulates expression multiple crucial functions The miR124-3p upregulated SC1-treated cells, targeting Grb2, Sos2 Egr1. Conclusion: enhances capacity modulating regulatory pluripotency-associated microRNAs. upregulates further inhibit MEK/ ERK